Ph Detection of Plant Cell Cultures by Using Tapered Fiber Probes

0350060 - ÚFE 2011 CZ eng A - Abstrakt
Kašík, Ivan - Martan, Tomáš - Pospíšilová, Marie - Podrazký, Ondřej - Mrázek, Jan - Matějec, Vlastimil
Ph Detection of Plant Cell Cultures by Using Tapered Fiber Probes.
EUROPT(R)ODE X. Vol. XConference on Optical Chemical Sensors and Biosensors. Praha: Institute of Photonics and Electronics AS CR, v.v.i, 2010 - (Homola, J.). s. 77-77. ISBN 978-80-86269-20-7.
[EUROPT(R)ODE X – X.Conference on Optical Chemical Sensors and Biosensors. 28.03.2010-31.3.2010, Praha]
Grant CEP: GA ČR(CZ) GP102/08/P639
Výzkumný záměr: CEZ:AV0Z20670512
Klíčová slova: tapered optical fiber * sensor * pH
Kód oboru RIV: JB - Senzory, čidla, měření a regulace

V-taper probes were prepared by tapering a stack of two polymer-clad-silica (PCS) fibers to tips with diameters in a range from 8 to 50 um. A fluorescence transducer 2’,7’-Bis(2- carbonylethyl)-5(6)-carboxyfluorescein (BCECF) was immobilized onto the end-face of the cut V-tapers by using a thin tetraethoxysilane layer. By using a PCS V-taper probe with a tip diameter of 44 μm excited by a multimode pigtailed source, extracellular pH was determined in real cell samples with the resolution of 0.2. Plant-cell walls were successfully penetrated with these probes.
Trvalý link: http://hdl.handle.net/11104/0190156