Supercritical Fluid Fractionation for 20-Hydroxyecdysone Enrichment in Leuzea carthamoides Extract

0347239 - ÚCHP 2011 CZ eng C - Konferenční příspěvek (zahraniční konf.)
Sajfrtová, Marie - Maščeník, J. - Sovová, Helena - Bártlová, Milena
Supercritical Fluid Fractionation for 20-Hydroxyecdysone Enrichment in Leuzea carthamoides Extract.
Summaries 2. Praha: Process Engineering Publisher, 2010, s. 609. ISBN 978-80-02-02247-3.
[International Congress of Chemical and Process Engineering CHISA 2010 and 7th European Congress of Chemical Engineering ECCE-7 /19./. Prague (CZ), 28.08.2010-01.09.2010]
Grant CEP: GA MŠMT 2B06024
Výzkumný záměr: CEZ:AV0Z40720504
Klíčová slova: supercritical fluid extraction * hplc analysis * plant
Kód oboru RIV: CI - Průmyslová chemie a chemické inženýrství
www.chisa.cz/2010, www.ecce7.com

Supercritical fluid extraction of 20-hydroxyecdysone (20-HE) using pure and modified carbon dioxide from seeds of Leuzea carthamoides was studied. The effect of operation conditions on the yields of whole extract and its minor component 20-HE was analysed in a series of experiments at pressures from 30 MPa to 60 MPa, temperatures from 40 °C to 80 °C and modifier (ethanol, methanol and acetone) concentrations in CO2 0-19 wt. %. The consecutively collected extract fractions were analysed using HPLC. The efficiency of SFE in terms of 20-HE concentration in extract and yield was compared with the efficiency of Soxhlet extraction with ethanol.
Trvalý link: http://hdl.handle.net/11104/0188059