Počet záznamů: 1
A lens-specific co-injection marker for medaka transgenesis
- 1.0346841 - ÚMG 2011 RIV US eng J - Článek v odborném periodiku
Vopálenský, Pavel - Růžičková, Jana - Pavlů, Barbora - Kozmik, Zbyněk
A lens-specific co-injection marker for medaka transgenesis.
Biotechniques. Roč. 48, č. 3 (2010), s. 235-236. ISSN 0736-6205. E-ISSN 1940-9818
Grant CEP: GA AV ČR IAA500520908; GA MŠMT 1M0520
Grant ostatní: GA UK(CZ) 91808
Výzkumný záměr: CEZ:AV0Z50520514
Klíčová slova: lens * transgenic * crystallin
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.550, rok: 2010
Recent advances in fish transgenesis allow generation of transgenic animals at high numbers and efficiency of germ-line transmission. To facilitate the procedure, a suitable co-injection marker may be very helpful for preselection of transgenic embryos. For this purpose, a lens-specific marker appears to be a suitable candidate since the lens is a well defined tissue positioned at the periphery and is easily accessible for examination of reporter gene expression. Here, we constructed reporter vectors based on mouse gamma-F crystallin (mF-Cry) promoter driving heterologous expression of the reporter gene at a high level and very specifically to the lens allowing an easy sorting of transgenics.
Trvalý link: http://hdl.handle.net/11104/0187759
Počet záznamů: 1