Extraction of water-soluble proteins from Bt-transgenic and non-transgenic maize species and tryptic digests peptide profiling by capillary electrophoresis

0343062 - ÚOCHB 2011 CZ eng C - Konferenční příspěvek (zahraniční konf.)
Sázelová, Petra - Kašička, Václav - Ibanez, E. - Cifuentes, A.
Extraction of water-soluble proteins from Bt-transgenic and non-transgenic maize species and tryptic digests peptide profiling by capillary electrophoresis.
Proceedings of the International Symposium Advances in Chromatography and Electrophoresis & Chiranal 2010. Olomouc: Palacký University Olomouc, 2010, s. 133-134. Acta Universitatis Palackianae Olomucensis Facultas Rerum Naturalium, Chemica, 47S. ISBN 978-80-244-2470-5.
[International Symposium Advances in Chromatography and Electrophoresis & Chiranal 2010. Olomouc (CZ), 08.02.2010-11.02.2010]
Grant CEP: GA ČR(CZ) GA203/08/1428; GA ČR(CZ) GA203/09/0675
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: capillary zone electrophoresis * Bt-transgenic maize * peptide mapping
Kód oboru RIV: CB - Analytická chemie, separace

Water-soluble proteins extracted from Bt-transgenic and non-transgenic maize varieties were digested by trypsin and analyzed by capillary zone electrophoresis (CZE) in several acidic background electrolytes. The found qualitative and quantitative differences among CZE profiles of tryptic peptide maps can be potentially used to differentiate transgenic and non-transgenic maize species.
Trvalý link: http://hdl.handle.net/11104/0185627