Electromigration techniques as a useful tool for the identification of similar strains of microorganisms

0341693 - ÚIACH 2011 CZ eng A - Abstrakt
Horká, Marie - Horký, J. - Kubesová, Anna - Mazanec, Karel - Matoušková, H. - Šlais, Karel
Electromigration techniques as a useful tool for the identification of similar strains of microorganisms.
MSB 2010. Final Program and Book of Abstracts. 25 th International Symposium on Microscale BioSeparations. Brno: Ústav analytické chemie AV ČR, v. v. i, 2010 - (Foret, F.). s. 147-148. ISBN 978-80-254-6631-5.
[International Symposium on Microscale BioSeparations /25./. 21.03.2010-25.03.2010, Praha]
Výzkumný záměr: CEZ:AV0Z40310501
Klíčová slova: capilary electrophoresis * pseudomonas * gas chromatography
Kód oboru RIV: CB - Analytická chemie, separace

The detection and identification of pathogens currently relies upon a very diverse range of techniques and skills, from traditional culturing and taxonomic procedures to modern molecular-based methods. The wide range of methods employed reflects the great diversity of microorganisms and the hosts they infect. In this study similar strains of different Pseudomonas syringae pathovars were identified by the gas chromatography of fatty acid methyl esters used in the routine laboratory and compared with results from the developed electromigration and mass spectrometry techniques. Acording to our experiments appropriate combination of electromigration techniques appear to be useful for fast detection the correct results rapidly and economicly.
Trvalý link: http://hdl.handle.net/11104/0184595