Fast estimation of modes of action of antimicrobials on yeast Saccharomyces cerevisiae - from MDR pump inhibition with a minimal effect on membrane potential up to cell permeabilizatio

0340980 - MBÚ 2010 RIV HR eng A - Abstrakt
Kodedová, M. - Drietomská, A. - Hendrych, T. - Sigler, Karel - Gášková, Dana
Fast estimation of modes of action of antimicrobials on yeast Saccharomyces cerevisiae - from MDR pump inhibition with a minimal effect on membrane potential up to cell permeabilizatio.
CESAR 2009. Zagreb: Pressum d.o.o., Zagreb, 2009. s. 38-38.
[Central European Symposium on Antimicrobial Resistance CESAR 2009. 23.09.2009-26.09.2009, Zadar]
Grant CEP: GA MŠMT 1M0570
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: mdr pump
Kód oboru RIV: EE - Mikrobiologie, virologie

Antimicrobial drugs that target directly the cell surface structures, resulting in cell permeabilization, were screened by our fluorescence method based on the use of the potentiometric fluorescent probe diS-C3(3). DiS-C3(3) is a substrate for two major S. cerevisiae MDR pumps, Pdr5p and Snq2p. Its use represents an efficient strategy for identifying pump inhibitors with minimal side effects on membrane potential or membrane integrity, and for detecting cells permeabilized by surface active drugs. The screening is performed on cells in well-defined physiological state and does not require any pretreatment or preincubation
Trvalý link: http://hdl.handle.net/11104/0184105