The effect of the mutations in the HsdS polypeptide on function of the Type I restriction-modification system EcoR124I

0340965 - MBÚ 2010 RIV GR eng A - Abstrakt
Guzanová, A. - Šišáková, Eva - Weiserová, Marie
The effect of the mutations in the HsdS polypeptide on function of the Type I restriction-modification system EcoR124I.
International Conference on Pathways, Networks, and Systems Medicine /7./. Corfu: Springer, 2009. s. 73-73.
[International Conference on Pathways, Networks, and Systems Medicine /7./. 06.06.2009-11.06.2009, Corfu]
Grant CEP: GA ČR GA204/07/0325
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: mutation
Kód oboru RIV: EE - Mikrobiologie, virologie

In the most complex Type I RM systems, restriction and modification activities are catalysed by one enzyme composed of three different subunits, which are encoded by the hsdR, hsdM and hsdS genes.The HsdS subunit plays a key role in the function of Type I R-M enzymes being responsible for both the specific recognition of the target DNA and interaction with HsdM and HsdR subunits, while the HsdR subunit is representing the motor component of this enzyme being responsible for ATP-binding, ATP- hydrolysis and subsequent DNA translocation. We focused on identification of amino acids residues involved in subunit assembly of the Type I R-M enzyme EcoR124I
Trvalý link: http://hdl.handle.net/11104/0184095