Conformational stabile p53 mutants retain specific DNA binding capacity but lost transactivation function

0333809 - BFÚ 2010 IL eng A - Abstrakt
Brázda, Václav - Brázdová Jagelská, Eva - Fojta, Miroslav
Conformational stabile p53 mutants retain specific DNA binding capacity but lost transactivation function.
[Konfirmačně stabilní kurantní formy proteinu p53 se mohou vázat sekvenčně specificky na DNA, ztrácí ale svoji transaktivační funkci.]
Abstract. Akko, 2009. s. 45.
[International p53 Marathon, 4th International Workshop on Mutant p53 „In vivo effects of mutant p53: experimental data, animal models, clinical consequences“. 17.03.2009-29.03.2009, Akko]
Grant CEP: GA ČR(CZ) GP301/07/P160
Výzkumný záměr: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Klíčová slova: p53 mutants * conformation of the mutant * MAbs
Kód oboru RIV: BO - Biofyzika

Our results show that p53 mutants R280K, E285K and L194F from human cancer cell lines were capable bind to p53 target sequences in vitro at 32oC or 4oC and the p53 binding ability is strongly improved by UV and Roscovitine, but also by cultivation at 32oC. Mutant E285K was moreover capable of reactivating the transcription of the reporter gene at 32oC in vivo while R285K and L194F were not. We observed changes in conformation of the mutant by MAbs. We conclude that conformation of p53 protein is only one of the preconditions in complex p53/DNA binding in vivo.

Byl sledován vliv teploty na vazbu mutantních forem proteinu p53 a schopnost zachování transaktivační a vazebné schopnosti různých p53 mutantních forem s ohledem na jejich konformační změny.
Trvalý link: http://hdl.handle.net/11104/0178713