Separation and characterization of GnRHs by capillary electrophoresis in classical and isoelectric buffers

0332508 - ÚOCHB 2011 CZ eng C - Konferenční příspěvek (zahraniční konf.)
Šolínová, Veronika - Poitevin, M. - Barth, Tomislav - Peltre, G. - Kašička, Václav
Separation and characterization of GnRHs by capillary electrophoresis in classical and isoelectric buffers.
Biologically Active Peptides. 11th Conference. Praha: Institut of organic Chemistry and Biochemistry ASCR, 2009 - (Slaninová, J.), s. 124-127. Collection Symposium Series, 11. ISBN 978-80-86241-31-9.
[Biologically Active Peptides. Conference /11./. Praha (CZ), 22.04.2009-24.04.2009]
Grant CEP: GA ČR(CZ) GA203/08/1428
Grant ostatní: GA ČR(CZ) GA203/09/0675
Program: GA
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: capillary zone electrophoresis * peptides * GnRH
Kód oboru RIV: CB - Analytická chemie, separace

Capillary zone electrophoresis (CZE) in classical buffer-based background electrolytes and carrier ampholytes-based capillary electrophoresis (CABCE) using isoelectric BGEs (narrow pH fractions of carrier ampholytes used in isoelectric focusing) have been applied to separation and characterization of synthetic human gonadotropin-releasing hormone (hGnRH) and its fragments. From the measured effective mobilities of these peptides within a broad pH range (3.0-10.0), their isoelectric points (pI) were determined.
Trvalý link: http://hdl.handle.net/11104/0177754