Enzymatic and inhibiting activity of epididymal fluid in pig

0329731 - BTÚ 2011 US eng A - Abstrakt
Davidová, Nina - Dorosh, Andriy - Jonáková, Věra - Postlerová, Pavla
Enzymatic and inhibiting activity of epididymal fluid in pig.
Biology of Reproduction 2009 Supplement. Madison: Society for the Study of Reproduction, 2009 - (Robaire, B.; Murphy, B.). s. 155. ISSN 0006-3363. E-ISSN 1529-7268.
[SSR 42nd Annual Meeting. 18.07.2009-22.07.2009, Pittsburgh]
Grant CEP: GA MŠMT(CZ) 1M06011; GA ČR(CZ) GA303/09/1285; GA ČR(CZ) GA523/08/HD64
Výzkumný záměr: CEZ:AV0Z50520701
Klíčová slova: sperm maturation * epididymal fluid * serine proteinase * mettaloproteinase * acrosin
Kód oboru RIV: CE - Biochemie

Sperm maturation takes place during the transit of spermatozoa through the epididymis. Sperm plasma membrane is exposed to epididymal fluid components essential for maturation. Changes in the sperm membrane proteins are influenced by proteolytic and glycosidic enzymes. Boar epididymal fluid was separated by size exclusion chromatography and by RP HPLC. The SDS-PAGE separated proteins were investigated for enzymatic and proteinase inhibiting activities by substrate and reverse substrate zymographies. By zymographic methods, we detected serine proteinases and metalloproteinases of 30, 40, 63 and 65 kDa, trypsin-like proteinase inhibitors of 6, 9, 18 and 21 kDa, and serine proteinase and metalloproteinase inhibitors with molecular masses of 12, 14, 17 and 60 kDa. The presence of acrosin inhibitor of 12 kDa and 8 kDa in epididymal fluid and spermatozoa was proved using specific antibody. Gene expression of acrosin inhibitor increased gradually throughout the epididymal duct.mettaloproteina
Trvalý link: http://hdl.handle.net/11104/0175682