Rapid-resolution HPLC with specgtrometric detection for the determination and identification of isoflavones in soy preparations and plant extracts

0314592 - MBÚ 2009 RIV DE eng J - Článek v odborném periodiku
Klejdus, B. - Vacek, J. - Benešová, L. - Kopecký, Jiří - Lapčík, O. - Kubáň, V.
Rapid-resolution HPLC with specgtrometric detection for the determination and identification of isoflavones in soy preparations and plant extracts.
[Rychlá HPLC se spectrofotometrickou detection for determination and identification of isoflavones in soy preparations and plant extracts.]
Analytical and Bioanalytical Chemistry. Roč. 389, - (2007), s. 2277-2285. ISSN 1618-2642. E-ISSN 1618-2650
Grant CEP: GA ČR GA525/07/0338; GA ČR GA525/06/0864
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: rapid resolution * fast chromatography * hplc
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.867, rok: 2007

Rapid-resolution HPLC/DAD/MS separation (in less then 1 min) was optimized for determination and identification of isoflavones in fmol quantities and submicrolitre sample volumes. Method was verified using real samples of soya, Trifolium pratense, Iresine herbstii and Ononis spinosa. Pseudobaptigenin glucoside, irilone, prunetin, texasin, tlatlancuayin and other isoflavones, in addition to aglycones of isoflavones and their -glucosides and malonyl- and acetyl- derivates, were identified in the extracts

Rychlá separace pomocí HPLC/DAD/MS (pod 1 min) byla optimalizována pro stanovení a identifikaci isoflavonů v rozsahu fmolů a v objemech pod jeden mikrolitr. Metoda byla ověřena na reálných vzorcích soji, Trifolium pratense, Iresine herbstii a Ononis spinosa. Kromě glykosidu pseudobaptigeninu, irilonu, prunetinu, texasinu, tlatlancuayinu a dalších isoflavonů byly v extraktech identifikovány i jejich aglykony, -glykosidy, malonyl- a acetyl- deriváty
Trvalý link: http://hdl.handle.net/11104/0165056