Investigation of nanoparticle coating by fluorescence correlation spectroscopy

0311010 - ÚFCH JH 2009 RIV DE eng J - Článek v odborném periodiku
Jurkiewicz, Piotr - Koňák, Čestmír - Šubr, Vladimír - Hof, Martin - Štěpánek, Petr - Ulbrich, Karel
Investigation of nanoparticle coating by fluorescence correlation spectroscopy.
[Výzkum nanočástic ve fluorescenční korelační spektroskopii.]
Macromolecular Chemistry and Physics. Roč. 209, č. 14 (2008), s. 1447-1453. ISSN 1022-1352. E-ISSN 1521-3935
Grant CEP: GA AV ČR IAA100500501; GA AV ČR IAA4050403; GA AV ČR KAN200200651
Výzkumný záměr: CEZ:AV0Z40400503; CEZ:AV0Z40500505
Klíčová slova: coatings * drug delivery systems * fluorescence * light scattering
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
Impakt faktor: 2.202, rok: 2008

Protecting gene delivery vectors by hydrophilic copolymer coating is a promising approach in targeted gene therapy. Herein we demonstrate the usefulness of FCS in characterizing the coating process of a virus model - latex beads with coumarin-labeled N-(2-hydroxypropyl)methacrylamide copolymer containing thiazolidine-2-thione groups. FCS gives an estimation of the average number of polymer chains coating the latex bead, which agrees with light scattering results, giving an evidence of quality of the vector surface modification. FCS is advantageous because it decreases the sample consumption by a factor of 1 000. The coumarin label showed advantages over the previously used fluorescein-based one.

Výzkum nanočástic ve fluorescenční korelační spektroskopii.
Trvalý link: http://hdl.handle.net/11104/0162733