Polymorphism and mapping of the porcine inhibitor of differentiation genes, ID1, ID2, ID3 and ID4

0310133 - ÚŽFG 2009 NL eng A - Abstrakt
Stratil, Antonín - Filkuková, Jitka - Horák, Pavel - Van Poucke, M. - Bartenschlager, H. - Peelman, L. J. - Geldermann, H.
Polymorphism and mapping of the porcine inhibitor of differentiation genes, ID1, ID2, ID3 and ID4.
[Polymorfismus a mapování genů ID1, ID2, ID3 and ID4 (porcine inhibitor of differentiation genes).]
XXXI Conference of the International Society for Animal Genetics. Amsterdam: RAI Conference Center, 2008. s. 2179-2179.
[Conference of the International Society for Animal Genetics/31./. 20.07.2008-24.07.2008, Amsterdam]
Grant CEP: GA ČR(CZ) GA523/06/1302
Výzkumný záměr: CEZ:AV0Z50450515
Klíčová slova: differentiation genes
Kód oboru RIV: EB - Genetika a molekulární biologie

ID proteins are important members of signaling pathways involved in development, cell cycle and tumorigenesis. They are considered negative regulators of MRFs and the responsible genes are therefore candidates for carcass and meat quality traits. Specific PCR primers for the four porcine ID genes (ID1, ID2, ID3 and ID4) were designed and the PCR fragments were comparatively sequenced (Meishan and Pietrain) to verify the genes and identify polymorphisms. The genes were mapped using radiation hybrid (IMpRH) panel and by linkage analysis in the Hohenheim Meishan x Pietrain F2 family. The results of RH mapping were: ID1 – SSC17, closest marker ENDO3 (21 cR; LOD=11.11); ID2 – SSC3, closest marker SW2532 (20 cR; LOD=13.88); ID3 – SSC6, closest marker SW709 (8.0 cR; LOD=16.10); ID4 – SSC7, closest marker SW1369 (65 cR; LOD=4.64). By linkage analysis only ID3 and ID4 genes were mapped (in ID1 and ID2 no suitable polymorphism was found). ID3 was mapped to SSC6 (…FABP3 – 3.5 cM – ID3 – 14.2 cM – S0146…) and ID4 to SSC7 (…S0064 – 27.9 cM – ID4 – 12.6 cM – CYP21A2…). The ID3 and ID4 genes are located in QTL regions for numerous carcass and meat quality traits. Significant associations were also found by direct analysis of ID3 and ID4 genotypes (GLM).

Geny byly mapovány pomocí radiačního hybridního panelu (IMpRH): ID1 – SSC17, nejbližší marker ENDO3 (21 cR; LOD=11.11); ID2 – SSC3, nejbližší marker SW2532 (20 cR; LOD=13.88); ID3 – SSC6, nejbližší marker SW709 (8.0 cR; LOD=16.10); ID4 – SSC7, nejbližší marker SW1369 (65 cR; LOD=4.64) a vazbového mapování: ID3 - SSC6 (…FABP3 – 3.5 cM – ID3 – 14.2 cM – S0146…) a ID4 - SSC7 (…S0064 – 27.9 cM – ID4 – 12.6 cM – CYP21A2…). Geny ID3 a ID4 jsou lokalizovány v QTL oblastech pro znaky jatečné hodnoty a kvality masa.
Trvalý link: http://hdl.handle.net/11104/0162081