0305981 - ÚEB 2008 RIV GB eng J - Článek v odborném periodiku
Potocký, Martin - Jones, M.A. - Bezvoda, R. - Smirnoff, N. - Žárský, Viktor
Reactive oxygen species produced by NADPH oxidase are involved in pollen tube growth.
[Reactive oxygen species produced by NADPH oxidase are involved in pollen tube growth.]
New Phytologist. Roč. 174, č. 4 (2007), s. 742-751. ISSN 0028-646X. E-ISSN 1469-8137
Grant CEP: GA MŠMT(CZ) LC06034
Výzkumný záměr: CEZ:AV0Z50380511
Zdroj financování: V - jiné veřejné zdroje
Klíčová slova: calcium signalling * cell expansion * hydrogen peroxide (H2O2)
Kód oboru RIV: EF - Botanika
Impakt faktor: 5.249, rok: 2007

Tip-localized reactive oxygen species (ROS) were detected in growing pollen tubes by chloromethyl dichlorodihydrofluorescein diacetate oxidation, while tip-localized extracellular superoxide production was detected by nitroblue tetrazolium (NBT) reduction. To investigate the origin of the ROS we cloned a fragment of pollen specific tobacco NADPH oxidase (NOX) closely related to a pollen specific NOX from Arabidopsis. Transfection of tobacco pollen tubes with NOX-specific antisense oligodeoxynucleotides (ODNs) resulted in decreased amount of NtNOX mRNA, lower NOX activity and pollen tube growth inhibition. The ROS scavengers and the NOX inhibitor diphenylene iodonium chloride (DPI) inhibited growth and ROS formation in tobacco pollen tube cultures. Exogenous hydrogen peroxide (H2O2) rescued the growth inhibition caused by NOX antisense ODNs. Exogenous CaCl2 increased NBT reduction at the pollen tube tip, suggesting that Ca2+ increases the activity of pollen NOX in vivo. The results show that tip-localized ROS produced by a NOX enzyme is needed to sustain the normal rate of pollen tube growth and that this is likely to be a general mechanism in the control of tip growth of polarized plant cells.

Reactive oxygen species produced by NADPH oxidase are involved in pollen tube growth.
Trvalý link: http://hdl.handle.net/11104/0159101