Počet záznamů: 1  

Evaluation of novel microtubules interfering agents myoseverin, tubulyzine and E2GG in primary cultures of rat hepatocytes

  1. 1.
    0305969 - ÚEB 2008 RIV SK eng J - Článek v odborném periodiku
    Dvořák, Z. - Modrianský, M. - Vrba, J. - Ulrichová, J. - Kryštof, Vladimír - Stýskala, Jakub - Pávek, P.
    Evaluation of novel microtubules interfering agents myoseverin, tubulyzine and E2GG in primary cultures of rat hepatocytes.
    [Evaluation of novel microtubules interfering agents myoseverin, tubulyzine and E2GG in primary cultures of rat hepatocytes.]
    General Physiology and Biophysics. Roč. 26, č. 3 (2007), s. 173-180. ISSN 0231-5882. E-ISSN 1338-4325
    Grant ostatní: Univerzita Palackého v Olomouci / Přírodovědecká fakulta(CZ) GP204/03/D231
    Výzkumný záměr: CEZ:AV0Z50380511
    Zdroj financování: V - jiné veřejné zdroje
    Klíčová slova: microtubule * tubulin polymerization * cytochrome P450
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 1.286, rok: 2007
    http://www.gpb.sav.sk/2007-3.htm

    We investigated the effects of novel microtubules interfering agents (MIAs) in primary cultures of rat hepatocytes. Cells were treated for 24 h with a known compound colchicine and newly synthesized derivatives myoseverin, tubulyzine, and E2GG. We examined the effects of MIAs on microtubules network integrity and on the polymerization capability of isolated tubulin. All tested MIAs inhibited microtubules assembly with the following IC50 values: tubulyzine (4.4 +/- 0.9 mu mol/1), myoseverin (7.0 +/- 0.8 mu mol/1), E2GG (16 +/- 2 mu mol/1), colchicine (2.0 +/- 0.4 mu mol/1). The potency of MIAs to perturb microtubular network integrity (monitored by immune-histochemistry) increased in the order tubulyzine < myoseverin < E2GG < colchicine. We described recently deleterious effects of MIAs on the expression of drug metabolizing enzymes, including CYPIAI Here we observed inhibitory effects of novel MIAs on dioxin-inducible expression of CYP1A1 mRNA in rat hepatocytes. We conclude that novel MIAs exert analogical biological response as classical MIAs such as colchicine or nocodazole. This further supports the hypothesis that tubulin is the primordial target of MIAs within the cell and that perturbation of microtubules dynamics and/or integrity triggers the biological effects described here.

    Evaluation of novel microtubules interfering agents myoseverin, tubulyzine and E2GG in primary cultures of rat hepatocytes.
    Trvalý link: http://hdl.handle.net/11104/0159090

     
     
Počet záznamů: 1  

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