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Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin?

  1. 1.
    0305964 - ÚEB 2008 RIV NL eng J - Článek v odborném periodiku
    Bairu, M. W. - Stirk, W. A. - Doležal, Karel - van Staden, J.
    Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin?
    [Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin?]
    Plant Cell, Tissue and Organ Culture. Roč. 90, č. 1 (2007), s. 15-23. ISSN 0167-6857. E-ISSN 1573-5044
    Grant CEP: GA ČR GA522/06/0108
    Výzkumný záměr: CEZ:AV0Z50380511
    Zdroj financování: V - jiné veřejné zdroje
    Klíčová slova: aromatic cytokinins * conservation * hyperhydricity
    Kód oboru RIV: ED - Fyziologie
    Impakt faktor: 1.068, rok: 2007

    Benzyladenine (BA) is the most widely used cytokinin in the micropropagation industry due to its effectiveness and affordability. It, however, has disadvantages such as genetic alteration and abnormal growth in some plants. Naturally occurring zeatin on the other hand is not as widely used as BA and is far more expensive. The use of meta-topolin and its derivatives as alternatives to BA and zeatin, both of which frequently have negative effects in tissue culture was investigated. In vitro grown Aloe polyphylla (an endangered medicinal and ornamental aloe) were cultured on full strength Murashige and Skoog basal medium with different concentrations of cytokinins and solidified with 1% Bacteriological Agar (Oxoid No. 1). mT was the preferred cytokinin both in terms of multiplication rate and rooting. The optimum concentration that induced regeneration and rooting is 5.0 mu M. The problem of hyperhydricity was totally controlled. Plants rooted spontaneously in multiplication medium, thus avoiding the extra rooting step of the protocol. More than 91% of the plants transferred to ex vitro conditions were successfully acclimatized.

    Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin.
    Trvalý link: http://hdl.handle.net/11104/0159085

     
     
Počet záznamů: 1  

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