Detection of foodborne pathogens using surface plasmon resonance biosensors

0303677 - URE-Y 20000090 FR eng A - Abstrakt
Brynda, Eduard - Homola, Jiří - Koubová, Vendula - Dostálek, Jakub - Rošický, Jiří - Škvor, J. - Rauch, P.
Detection of foodborne pathogens using surface plasmon resonance biosensors.
[Villeurbanne]: [Université Claude Bernard Lyon], 2000. Europt(r)ode V. Opt(r)ode 2000. Book of abstracts. s. 163-164
[European Conference on Optical Chemical Sensors and Biosensors EUROPT(R)ODE /5./. 16.04.2000-19.04.2000, Lyon-Villeurbanne]
Grant CEP: GA ČR GA102/99/0549
Výzkumný záměr: CEZ:AV0Z2067918
Klíčová slova: optical sensors * surface plasmons * biosensors
Kód oboru RIV: JB - Senzory, čidla, měření a regulace

Salmonella enteritidis and Listeria monocytogenes were detected in real time using a surface plasmon resonance (SPR) sensor with ATR spectral interrogation. The respective antibodies against the pathogens were immobilised on the gold sensor surface as a covalently crosslinked double layer or covalently bound on a crosslinked albium layer. Salmonella and Listeria could be detected by the sensor at concentrations down to 10.sup.6.sup.. The sensor sensitivity was comparable with that of standard ELISA.
Trvalý link: http://hdl.handle.net/11104/0113864