TCR delta gene is frequently rearranged in adult B lymphocytes

0191566 - UMG-J 20033067 RIV US eng J - Článek v odborném periodiku
Krejčí, O. - Prouzová, Z. - Horváth, Ondřej - Trka, J. - Hrušák, O.
TCR delta gene is frequently rearranged in adult B lymphocytes.
Journal of Immunology. Roč. 171, č. 2 (2003), s. 524-527. ISSN 0022-1767. E-ISSN 1550-6606
Výzkumný záměr: CEZ:AV0Z5052915
Klíčová slova: TCR * B lymphocyte * gene rearrangement
Kód oboru RIV: EC - Imunologie
Impakt faktor: 6.702, rok: 2003

TCR gene rearrangement generates diversity of T lymphocytes by V(D)J recombination. Ig genes are rearranged in B cells using the same enzyme machinery. TCR gene is postulated to rearrange exclusively in T lineage, but malignant B precursor lymphoblasts contain rearranged TCR genes in most patients. In this study we show that incomplete TCR delta rearrangements V2-D3 and D2-D3 occur each in up to 16% alleles in B lymphocytes of all healthy donors studied, but complete VDJ rearrangement was negative at the sensitivity limit of 1%. Data are based on real-time quantitative PCR validated by PAGE and sequencing of the cloned products. Therefore, TCR genes rearrange not exclusively in T lineage. This study opens up further questions regarding the exact extent of the "cross-lineage" TCR or Ig rearrangements in normal lymphocytes, specific subsets in which the cross-lineage rearrangements occur, and the physiological importance of these rearrangements.
Trvalý link: http://hdl.handle.net/11104/0087309