0191177 - UMG-J 20010246 CZ eng A - Abstrakt
Tuháčková, Zdena - Šloncová, Eva - Hlaváček, Jan - Sovová, Vlasta - Velek, Jiří
Activation of the protein kinase B and glycogen synthase kinase-3 signalling pathway during transient differentiation of human colon cancer HT-29 cells.
Collection of Czechoslovak Chemical Communications. Ústav organické chemie a biochemie AV ČR, v. v. i. Roč. 3, - (1999), s. 19-22. ISSN 0010-0765.
[Biologically Active Peptides /6./. 21.04.1999-23.04.1999, Praha]
Grant CEP: GA MZd IZ3517; GA ČR GA312/97/1188; GA ČR GV312/96/K205
Výzkumný záměr: CEZ:AV0Z5052915
Kód oboru RIV: EB - Genetika a molekulární biologie

Using synthetic peptides which reproduce phosphoacceptor sites in the molecule of GSK-3, PKB an eIF2B, we found that phosphorylation and activity of PKB increased very early during in vitro differentiation induced by sodium butyrate. This was inhibited by wortmannin, the selective inhibitor of phosphoinositol-3 kinase, indicating that the control of PI3 kinase/PKB/GSK-3 signalling pathway is the early event of transient differentiation of the human colon cancer cells HT-29.
Trvalý link: http://hdl.handle.net/11104/0086934