Cloning and characterisation of chs-specific DNA and cDNA sequences from hop (Humulus lupulus L.)

0189900 - UMBR-M 20023006 RIV IE eng J - Článek v odborném periodiku
Matoušek, Jaroslav - Novák, Petr - Bříza, Jindřich - Patzak, J. - Niedermeierová, Hana
Cloning and characterisation of chs-specific DNA and cDNA sequences from hop (Humulus lupulus L.).
Plant Science. Roč. 62, - (2002), s. 1007-1018. ISSN 0168-9452. E-ISSN 1873-2259
Grant CEP: GA ČR GA521/99/1591
Klíčová slova: hop * genetic manipulation
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 1.556, rok: 2002

A complete sequence of chalcone synthase (CHS) gene from hop was cloned. The gene designated chs_H1 consists of two exons and one 187 bp intron. CHS protein predicted from chs_H1 cDNA has 42.5 kDa and retains conserved domains and residues including 26 amino acids at positions identical to those identified by crystallography as characteristic for catalytic domains of alfalfa CHS (EC 2.3.1.74). Cloned CHS_H1 protein shows specific CHS activity with 4-coumaroyl-CoA.
Trvalý link: http://hdl.handle.net/11104/0085697