Recent Research Developments in Proteins

0181670 - UFCH-W 20030097 RIV IN eng M - Část monografie knihy
Hutterer, R. - Hof, Martin
Membrane Binding of Peripheral Proteins and its Characterisation Applying Fluorescence Methods.
Recent Research Developments in Proteins. Trivandrum: Transworld Research Network, 2002 - (Pandalai, S.), s. 277-288. ISBN 81-7895-063-4
Grant CEP: GA MŠMT LN00A032
Výzkumný záměr: CEZ:AV0Z4040901
Klíčová slova: membrane binding * proteins * fluorescence
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie

Basically, the problem of membrane binding of peripheral proteins can be studied in two ways. On the one hand we can address the protein itself, using either intrinsic tryptophan fluorescence or dyelabelled protein, as typically employed in fluorescence recovery after photobleaching experiments. On the other hand we can approach the binding event from the membrane side, studying solvent relaxation, fluorescence anisotropy or excimer formation of suitable membrane probes. For the case of the blood coagulation protein prothrombin and its fragment l the combined use of these fluorescence methods shows a slightly tighter binding of the fragment l portion in the case of the entire prothrombin molecule compared to the isolated fragment l while no evidence could be obtained for hydrophobic membrane binding sites in the ''non-fragment l'' part of prothrombin.
Trvalý link: http://hdl.handle.net/11104/0078204