ABA-C15: A New Dye for Probing Solvent Relaxation in Phospholipid Bilayers

0181554 - UFCH-W 20020186 RIV US eng J - Článek v odborném periodiku
Sýkora, Jan - Mudogo, V. - Hutterer, R. - Nepraš, M. - Vaněrka, J. - Kapusta, P. - Fidler, V. - Hof, Martin
ABA-C15: A New Dye for Probing Solvent Relaxation in Phospholipid Bilayers.
Langmuir. Roč. 18, č. 24 (2002), s. 9276-9282. ISSN 0743-7463
Výzkumný záměr: CEZ:AV0Z4040901
Klíčová slova: solvent relaxation * phospholipid bilayers * ABA-C15
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
Impakt faktor: 3.248, rok: 2002

We synthesized and studied N-palmitoyl-3-aminobenzanthrone (ABA-C15), which we proved to be an advantageous new fluorescent phospholipid membrane label. While the absorption of ABA-C15 in protic solvents shows negative solvatochromism, its fluorescence emission is substantially red-shifted when the polarity of the solvent is increased. ABA-C15 is excitable by lasers emitting in the range between 390 and 490 nm; it exhibits reasonable quantum yields in protic solvents and binds with high affinity to small unilamellar phospholipid vesicles. Absorption, steady state fluorescence, and solvent relaxation data indicate that the aminobenzanthrone chromophore is located in the headgroup region of phospholipid bilayers in the liquid crystalline state of small unilamellar vesicles. The solvent relaxation kinetics probed by ABA-C15 in the liquid crystalline state is characterized by three solvent relaxation times in the order of 0.05, 0.2, and 1.5 ns, respectively.
Trvalý link: http://hdl.handle.net/11104/0001906