Cell cycle dependent expression of Plk 1 in synchronized porcine fetal fibroblasts

0173866 - UEM-P 20033059 RIV US eng J - Článek v odborném periodiku
Anger, M. - Kues, W. A. - Klíma, J. - Mielenz, M. - Kubelka, M. - Motlík, J. - Ešner, M. - Dvořák, Petr - Carnwath, J. W. - Niemann, H.
Cell cycle dependent expression of Plk 1 in synchronized porcine fetal fibroblasts.
Molecular Reproduction and Development. Roč. 65, - (2003), s. 245-253. ISSN 1040-452X. E-ISSN 1098-2795
Grant CEP: GA MŠMT LN00A065
Výzkumný záměr: CEZ:AV0Z5039906
Klíčová slova: serum deprivation
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.543, rok: 2003

Enzymes of the Polo-like kinase family are active in the pathways controlling mitosis in several species. CDNA fragments of the porcine homologues of Plk1, Plk2, and Plk3 were cloned. All three cDNAs show high sequence homology with their mouse and human counterparts and contain the Polo box. In synchronized porcine fetal fibroblasts, Plk1 mRNA was barely detectable in G0 and G1, increased during S phase and peaked after G2/M transition. Porcine Plk1 protein was detected at the beginning of S phase and was highest after the G2/M transition. In summary, the Plk1 expression pattern in the pig is similar to that reported for other species.
Trvalý link: http://hdl.handle.net/11104/0070892