0172389 - UEB-Q 20033097 RIV NL eng J - Journal Article
Laukens, K. - Lenobel, René - Strnad, Miroslav - Onckelen, H. - Witters, E.
Cytokinin affinity purification and identification of a tobacco BY-2 adenosine kinase.
FEBS Letters. Roč. 533, 1/3 (2003), s. 63-66. ISSN 0014-5793. E-ISSN 1873-3468
R&D Projects: GA ČR GA301/02/0475
Grant - others:Scientific Research Flanders(BE) NOI BOF UA 2002
Institutional research plan: CEZ:AV0Z5038910
Keywords : Adenosine kinase * Tobacco Bright Yellow 2 * Cytokinin
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.609, year: 2003

Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be disrupted by several other adenine- but not guanine-based purine derivatives. The observed interaction with cytokinins is discussed in view of a putative role for adenosine kinase in TBY-2 cytokinin metabolism. The presented results show for the first time a plant adenosine kinase affinity-purified to homogeneity that was identified by primary structure analysis.
Permanent Link: http://hdl.handle.net/11104/0069428