Počet záznamů: 1  

Comparative Analysis of the Arabidopsis Pollen Transcriptome

  1. 1.
    0172314 - UEB-Q 20033039 RIV GB eng J - Článek v odborném periodiku
    Honys, David - Twell, D.
    Comparative Analysis of the Arabidopsis Pollen Transcriptome.
    Plant Physiology. Roč. 132, - (2003), s. 640ů652. ISSN 0032-0889. E-ISSN 1532-2548
    Grant CEP: GA AV ČR IAA5038207
    Grant ostatní: Royal Society(GB) NATO Postdoctoral Fellowship (to D.H.)
    Výzkumný záměr: CEZ:AV0Z5038910; CEZ:MSM 113100003
    Klíčová slova: transcriptome profiling * Arabidopsis pollen * male gametophyte
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 5.634, rok: 2003

    We present a genome-wide view of the male gametophytic transcriptome in Arabidopsis based on microarray analysis. In comparison with the transcriptome of the sporophyte throughout development, the pollen transcriptome showed reduced complexity and a unique composition. We identified 992 pollen-expressed mRNAs, nearly 40% of which were detected specifically in pollen. Analysis of the functional composition of the pollen transcriptome revealed the over-representation of mRNAs encoding proteins involved in cell wall metabolism, cytoskeleton, and signaling and under-representation of mRNAs involved in transcription and protein synthesis. For several gene families, we observed a common pattern of mutually exclusive gene expression between pollen and sporophytic tissues for different gene family members. Our results provide a 50-fold increase in the knowledge of genes expressed in Arabidopsis pollen. Moreover, we also detail the extensive overlap (61%) of the pollen transcriptome with that of the sporophyte, wich provides ample potential to influence sporophytic fitness through gametophytic selection.
    Trvalý link: http://hdl.handle.net/11104/0069353

     
     

Počet záznamů: 1  

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