Flow kaiyotyping and chromosome sorting in bread wheat (Triticum aestivum L)

0172237 - UEB-Q 20023014 RIV DE eng J - Článek v odborném periodiku
Doleželová, Marie - Vrána, Jan - Čihalíková, Jarmila - Šimková, Hana - Doležel, Jaroslav
Flow kaiyotyping and chromosome sorting in bread wheat (Triticum aestivum L).
Theoretical and Applied Genetics. Roč. 104, - (2002), s. 1362-1372. ISSN 0040-5752. E-ISSN 1432-2242
Grant CEP: GA AV ČR IAA6038204; GA AV ČR IBS5038104
Výzkumný záměr: CEZ:AV0Z5038910
Klíčová slova: Chromosome isolation * Chromosome sorting * Flow cytometry
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.264, rok: 2002

Previously, we reported on the development of procedures for chromosome analysis and sorting using flow cytometry (flow cytogenetics) in bread wheat. That study indicated the possibility of sorting large quantities of intact chromosomes, and their suitability for analysis at the molecular level. However, due to the lack of sufficient differences in size between individual chromosomes, only chromosome 3B could be sorted into a high-purity fraction. The present study aimed to identify wheat stocks that could be used to sort other chromosomes. An analysis of 58 varieties and landraces demonstrated a remarkable reproducibility and sensitivity of flow cytometry for the detection of numerical and structural chromosome changes. Changes in flow karyotype, diagnostic for the presence of the 1BL-1RS transloca-tion, have been found and lines from which translocation chromosomes 5BL-7BL and 4AL-4AS-5BL could be sorted have been identified. Furthermore, wheat lines have been identified which can be used for sorting chromosomes 4B, 4D, 5D and 6D.
Trvalý link: http://hdl.handle.net/11104/0069276