DNA damage induced by hydrogen peroxide in cultured tobacco cells is dependent on the cell growth stage

0172219 - UEB-Q 20023001 RIV NL eng J - Článek v odborném periodiku
Stavreva, D. - Gichner, Tomáš
DNA damage induced by hydrogen peroxide in cultured tobacco cells is dependent on the cell growth stage.
Mutation Research - Genetic Toxicology and Environmental Mutagenesis. Roč. 514, - (2002), s. 147-152. ISSN 1383-5718. E-ISSN 1879-3592
Grant CEP: GA ČR GA521/02/0400
Výzkumný záměr: CEZ:AV0Z5038910
Klíčová slova: DNA * peroxide * tobacco
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 1.636, rok: 2002

The level of hydrogen peroxide (HaOaMnduced genomic DNA damage measured by the Comet assay in tobacco suspension cells (TX1) increased as a function of the age of the culture. After treatment of TX1 cells with 15 mM H2O2, the average (+-S.E.) median tail moment value was only 4.85 +- 1.00 'xm in nuclei isolated from 2-day-old cells compared to 72.33 +- 1.40 (xm in nuclei isolated from 12-day-old cells. By contrast, nuclei first isolated from 2 and 12-day-old cells and then treated with HfeC, expressed the same level of DNA damage. The activity of catalases was markedly higher in 2-day-old TX1 cells compared to 12-day-old cells. The results indicate that the reaction of the H2O2 with nuclear DNA is modified by the presence of the plant cell wall, and enzymes and macromolecules present in the cytosol, and is not connected with changes in the nuclear DNA sensitivity during cell suspension growth.
Trvalý link: http://hdl.handle.net/11104/0069258