Microdevice for separation and quantitative fraction collection

0162507 - UIACH-O 20033070 RIV DE eng J - Článek v odborném periodiku
Spěšný, Michal - Foret, František
Microdevice for separation and quantitative fraction collection.
Electrophoresis. Roč. 24, č. 21 (2003), s. 3745-3747. ISSN 0173-0835. E-ISSN 1522-2683
Grant CEP: GA AV ČR IBS4031209
Výzkumný záměr: CEZ:AV0Z4031919
Klíčová slova: microfluidic system * miniaturization * whole-column fraction collection
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 4.040, rok: 2003

A new microfluidic concept for quantitative whole-column fraction collection of electrophoretically separated zones was developed. The prototype device, fabricated on a polycarbonate disk by injection molding, integrated electrophoretic separation channels with fraction collection reservoirs distributed along the separation channel. The microdevice was designed in a CD-like format to use the centrifugal force for moving the liquid in the microchannels. A serpentine shape of the separation channel was selected to create segments for quantitative whole-columnfraction collection. The operation was tested with visual monitoring of isotachophoretic separation and collection of cationic dyes.
Trvalý link: http://hdl.handle.net/11104/0059816