A miniaturized multichamber solution isoelectric focusing device for separation of protein digests

0162432 - UIACH-O 20020084 RIV DE eng J - Článek v odborném periodiku
Tan, A. - Pashkova, A. - Zang, L. - Foret, František - Karger, B. L.
A miniaturized multichamber solution isoelectric focusing device for separation of protein digests.
Electrophoresis. Roč. 23, č. 20 (2002), s. 3599-3607. ISSN 0173-0835. E-ISSN 1522-2683
Grant CEP: GA AV ČR IBS4031209
Výzkumný záměr: CEZ:AV0Z4031919
Klíčová slova: solution IEF * peptides * mass spectrometry
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 4.325, rok: 2002

A miniaturized multichamber device was constructed for solution isoelectric focusing (IEF) separation of complex peptide mixtures. A variety of standard peptide mixtures and tryptic digests of proteins were separated. The IEF separation provided concentrated and desalted fractions, suitable for an additional separation liquid chromatography, capillary electrophoresis (LC, CE) or mass spectrometry (MS) detection without additional sample cleanup.
Trvalý link: http://hdl.handle.net/11104/0059747