0162276 - UIACH-O 20010022 RIV US eng J - Článek v odborném periodiku
Radko, S. P. - Šťastná, Miroslava - Chrambach, A.
Polydispersity of liposome preparations as a likely source of peak width in capillary zone electrophoresis.
Journal of Chromatography-B. Roč. 761, č. 1 (2001), s. 69-75. ISSN 0378-4347
Výzkumný záměr: CEZ:AV0Z4031919
Klíčová slova: polydispersity * liposomes
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 1.911, rok: 2001

Negatively charged liposomes consisting of phosphatidylcholine/phosphatidylglycerol/cholesterol in various ratios when subjected to capillary zone electrophoresis (CZE) in Tris-HCl (pH 8) buffer of different concentrations have been shown previously to exhibit a size-dependent migration rate at low ionic strength. The present study, focusing on the peak width under those conditions, shows that the polydispersity of liposomes correlated with, and appears to be a dominant source of, the peak width of the liposomes in CZE in a buffer of low ionic strength (2 to 5 mM Tris-HCl buffer, pH 8) at moderate electric field strengths (200 V cm(-1) or less). This finding, beyond allowing for the analysis of liposome polydispersity by CZE, suggests that the size-dependent fractionation of liposome preparations by a preparative electrophoretic technique such as free-flow electrophoresis is potentially feasible.
Trvalý link: http://hdl.handle.net/11104/0059595