Two temperature-sensitive mutations in the DNA binding subunit of EcoKI with differing properties

0153890 - MBU-M 20013279 RIV NL eng J - Článek v odborném periodiku
Janščák, Pavel - Weiserová, Marie - Hubáček, Josef - Holubová, Inge - Dutta, CH. F. - Firman, K.
Two temperature-sensitive mutations in the DNA binding subunit of EcoKI with differing properties.
FEMS Microbiology Letters. Roč. 182, č. 1 (2000), s. 99-104. ISSN 0378-1097. E-ISSN 1574-6968
Grant CEP: GA ČR GA204/96/1365
Výzkumný záměr: CEZ:AV0Z5020903
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 1.615, rok: 2000

Two temperature-sensitive mutations in the hsdS gene, which encodes the DNA specificity subunit of the type IA restriction-modification system EcoKI, designated Sts1 and Sts2 had a different impact on restriction-modification functions in vitro and in vivo. The enzyme activities of the Sts1 mutant temperature-sensitive in vitro and were reduced even at 30 o C (permissive temperature).
Trvalý link: http://hdl.handle.net/11104/0051382