Secondary and tertiary structure of nucleotide-binding domain of alpha subunit of Na+/K+-ATPase

0142289 - FGU-C 20020010 RIV US eng J - Článek v odborném periodiku
Hofbauerová, Kateřina - Kopecký ml., Vladimír - Ettrich, Rüdiger - Ettrichová, Olga - Amler, Evžen
Secondary and tertiary structure of nucleotide-binding domain of alpha subunit of Na+/K+-ATPase.
Biopolymers. Roč. 67, 4-5 (2002), s. 242-246. ISSN 0006-3525. E-ISSN 1097-0282
Grant CEP: GA ČR GA204/01/0254; GA ČR GA204/01/1001
Grant ostatní: Volkswagen Foundation(DE) I/74 679
Výzkumný záměr: CEZ:AV0Z5011922
Klíčová slova: Na+/K+-ATPase * ATP binding * molecular modeling
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 2.372, rok: 2002

The nucleotide-binding domain of the alpha subunit of mouse brain Na+/K+-ATPase was expressed and isolated from Escherichia coli cells. A model structure was constructed by comparative modeling with and without docked ATP. This was compared with the secondary structure determination from UV circular dichroism and Raman spectroscopy. Thus, we support the quality of the model and the correct folding of the recombinant protein. ATP binding was followed by Raman difference spectroscopy, and its influence on the secondary structure of the N domain seems to not be significant.
Trvalý link: http://hdl.handle.net/11104/0039995