Three-dimensional study of the capillary supply of skeletal muscle fibres using confocal microscopy

0142221 - FGU-C 20010280 RIV NL eng J - Článek v odborném periodiku
Kubínová, Lucie - Janáček, Jiří - Ribarič, S. - Čebašek, V. - Eržen, I.
Three-dimensional study of the capillary supply of skeletal muscle fibres using confocal microscopy.
Journal of Muscle Research and Cell Motility. Roč. 22, č. 3 (2001), s. 217-227. ISSN 0142-4319. E-ISSN 1573-2657
Grant CEP: GA AV ČR IAA5011810; GA MŠMT ME 256; GA ČR GA304/01/0257
Výzkumný záměr: CEZ:AV0Z5011922
Klíčová slova: capillaries * confocal microscopy * stereology
Kód oboru RIV: EA - Morfologické obory a cytologie
Impakt faktor: 1.460, rok: 2001

Three-dimensional study of capillary network of individual muscle fibres in rat extensor digitorum longus (EDL) and soleus (SOL) muscles is presented. Stereology and three-dimensional reconstruction techniques were applied to stacks of serial optical sections recorded by a confocal microscope from thick muscle slices. The results suggest that SOL muscle fibres have a larger surface area and volume as well as a larger length of capillaries per fibre length than EDL. On the other hand, these two muscles have a similar ratio of capillary length to fibre surface area. The three-dimensional approach to evaluation of muscle fibre capillarization brings many advantages over traditional measurements made on single muscle sections and could also be applied to the study of angiogenesis in other tissues.
Trvalý link: http://hdl.handle.net/11104/0039928