Differentiation of NG108-15 cells induced by the combined presence of dbcAMP and dexamethasone brings about the expression of N and P/Q types of calcium channels and the inhibitory influence of muscarinic receptors on calcium influx

0142136 - FGU-C 20010190 RIV NL eng J - Článek v odborném periodiku
Doležal, Vladimír - Lisá, Věra - Diebler, M. F. - Kašparová, Jana - Tuček, Stanislav
Differentiation of NG108-15 cells induced by the combined presence of dbcAMP and dexamethasone brings about the expression of N and P/Q types of calcium channels and the inhibitory influence of muscarinic receptors on calcium influx.
Brain Research. Roč. 910, 1-2 (2001), s. 134-141. ISSN 0006-8993. E-ISSN 1872-6240
Grant CEP: GA ČR GA305/98/0469; GA ČR GA305/01/0283; GA MZd NF5183
Výzkumný záměr: CEZ:AV0Z5011922
Klíčová slova: NG108-15 cell line * cAMP * calcium channels
Kód oboru RIV: ED - Fyziologie
Impakt faktor: 2.489, rok: 2001

Differentiation of cholinergic cell line NG108-15 induced by a combination of dibutyryl cyclic AMP (dbcAMP) and dexamethasone enhances the cholinergic phenotype of the cells more than that induced by either agent alone. We investigated the effect of treatment with dbcAMP and dexamethasone on potassium depolarization-evoked influx of calcium and its regulation by the muscarinic agonist carbachol. Depolarization of control cells and of cells differentiated in the presence of dbcAMP or dexamethasone alone, or in the combined presence of dbcAMP and dexamethasone induced, respectively, 2.2, 4.3, 2.7 and 10.7 fold increases of the resting [Ca2+]i. Dexamethasone alone and the combination of dbcAMP and dexamethasone augmented the number of muscarinic receptors by 25 and 40
Trvalý link: http://hdl.handle.net/11104/0039845