A quantitative study of adipokinetic hormone of the firebug Pyrrhocoris apterus

0135864 - ENTU-I 20023083 RIV GB eng J - Článek v odborném periodiku
Goldsworthy, G. J. - Kodrík, Dalibor - Comley, R. - Lightfoot, M.
A quantitative study of adipokinetic hormone of the firebug Pyrrhocoris apterus.
Journal of Insect Physiology. Roč. 48, - (2002), s. 1103-1109. ISSN 0022-1910. E-ISSN 1879-1611
Grant CEP: GA AV ČR IAA6007202
Výzkumný záměr: CEZ:AV0Z5007907
Klíčová slova: Adipokinetic hormone * ELISA * Pyrrhocoris apterus
Kód oboru RIV: ED - Fyziologie
Impakt faktor: 1.789, rok: 2002

The development of an enzyme-linked immunoassay (ELISA) for the adipokinetic neuropeptide hormone, Pya-AKH, from the firebug Pyrrhocoris apterus L. is described. The ELISA measures as little as 20 fmol of Pya-AKH. Tested against a range of synthetic peptides, the assay has a high sensitivity for peptides containing the C-terminal motif FTPNWamide. The amounts of Pya-AKH in the brain, corpora cardiaca, suboesophageal ganglia, and fused thoracic and abdominal ganglionic mass are very small, with only the corpora cardiaca containing appreciable levels of the hormone (ca. 4pmol per bug).
Trvalý link: http://hdl.handle.net/11104/0033640