Automated microaxial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation

0127045 - BFU-R 20033133 RIV GB eng J - Článek v odborném periodiku
Kozubek, Michal - Skalníková, M. - Matula, Pe. - Bártová, Eva - Rauch, J. - Neuhaus, F. - Eipel, H. - Hasmann, M.
Automated microaxial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation.
Micron. Roč. 33, 7-8 (2002), s. 655-665. ISSN 0968-4328. E-ISSN 1878-4291
Výzkumný záměr: CEZ:AV0Z5004920
Klíčová slova: microaxial tomography * automated microscopy * high-resolution cytometry
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 1.537, rok: 2002

Microaxial tomography provides a good means for microscopic image acquisition of cells or sub-cellular components like cell nuclei with an improved resolution. because shortcomings of spatial resolution anisotropy in optical microscopy can be overcome. Thus, spatial information of the object can be obtained without the necessity of confocal imaging. Since the very early developments of microaxial tomography, a considerable drawback of this method was a complicated image acquisition and processing procedure that requires much operator time. In order to solve this problem the Heidelberg 2pi-tilting device has been mounted on the Brno high-resolution cytometer as an attempt to bring together advanced microscopy and fast automated computer image acquisition and analysis. A special software module that drives all hardware components required for automated microaxial tomography and performs image acquisition and processing has been developed.
Trvalý link: http://hdl.handle.net/11104/0025269