Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation

0126886 - BFU-R 20023186 RIV CZ eng J - Článek v odborném periodiku
Pacherník, Jiří - Janík, Robert - Hofmanová, Jiřina - Bryja, Vítězslav - Kozubík, Alois
Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation.
Folia Biologica. Roč. 48, č. 4 (2002), s. 154-156. ISSN 0015-5500. E-ISSN 0015-5500
Grant CEP: GA ČR GA524/99/0694; GA AV ČR IBS5004009; GA AV ČR KSK5011112
Výzkumný záměr: CEZ:AV0Z5004920
Klíčová slova: leukaemia cell line HL-60 * electroporation * gene transfection
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 0.615, rok: 2002

Electroporation represents a powerful technique for cell transfection; however, its efficiency in haemopoietic cells (~1%) is largely unsatisfactory. Biological processes in haemopoietic cells are often studied using leukemia cell line HL-60. For this reason we developed conditions for efficiently introducing plasmids to HL-60 cells by electroporation, as an alternative to other techniques. This technique employs electric pulse (250-270 V; 1000 microF) followed by separation of living cells on Ficoll-Paque discontinuous gradient. Using 10-20 microg of plasmid, we routinely achieve 12-14% of transfectants.
Trvalý link: http://hdl.handle.net/11104/0025113