Hypomethylation of CNG targets induced with dihydroxypropyladenine is rapidly reversed in the course of mitotic cell division in tobacco

0126876 - BFU-R 20023176 RIV DE eng J - Článek v odborném periodiku
Koukalová, Blažena - Votruba, Ivan - Fojtová, Miloslava - Holý, Antonín - Kovařík, Aleš
Hypomethylation of CNG targets induced with dihydroxypropyladenine is rapidly reversed in the course of mitotic cell division in tobacco.
Theoretical and Applied Genetics. Roč. 105, č. 5 (2002), s. 796-801. ISSN 0040-5752. E-ISSN 1432-2242
Grant CEP: GA ČR GA521/01/0037; GA ČR GP521/01/P042
Výzkumný záměr: CEZ:AV0Z5004920
Klíčová slova: plant DNA methylation * S-adenosylhomocysteine hydrolase inhibition * CNG and CG methylation motifs
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 2.264, rok: 2002

We followed the mitotic transmission of an experimentally induced hypomethylated state of several tobacco repetitive sequences in callus culture and plants. The initial hypomethylation was induced by a hypomethylation drug, dihydroxypropyladenine (DHPA), the competitive inhibitor of cellular S-adenosylhomocysteine hydrolase, which is known to preferentially inhibit methylation at CNG and non-symmetrical motifs while having a negligible effect on methylation at CG motifs. The deprivation of this drug resulted in an almost immediate remethylation of cytosines at CNG motifs (MspI and EcoRII sites) leading us to conclude that, the hypomethylation effect of dihydroxypropyladenine is rather transient and differs from that of 5-azacytidine which often induces heritable changes in methylation patterns. The results suggest that de novo methylation of CNG motifs is a rapid and meiotically independent process on DNA sequences with pre-existing CG methylation.
Trvalý link: http://hdl.handle.net/11104/0025103