Topography of genetic elements of X-chromosome relative to the cell nucleus and to the chromosome X territory determined for human lymphocytes

0126868 - BFU-R 20023168 RIV NL eng J - Článek v odborném periodiku
Falk, Martin - Lukášová, Emilie - Kozubek, Stanislav - Kozubek, Michal
Topography of genetic elements of X-chromosome relative to the cell nucleus and to the chromosome X territory determined for human lymphocytes.
Gene. Roč. 292, 1-2 (2002), s. 13-24. ISSN 0378-1119. E-ISSN 1879-0038
Grant CEP: GA AV ČR IBS5004010; GA MZd NC5955; GA ČR GA202/01/0197; GA ČR GA301/01/0186
Výzkumný záměr: CEZ:AV0Z5004920
Klíčová slova: structure of chromosome X territories * chromatin condensation * nuclear topology
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 2.778, rok: 2002

Topography of three genetic elements - dystrophin (dmd) exons 5-7 (E1), 46-47 (E2), and centromere of chromosome X (NX) were studied relative to cell nuclei and to chromosome X territories of spatially fixed human lymphocytes. Repeated three-dimensional (3D) dual color fluorescence in situ hybridization (FISH) combined with high-resolution cytometry was used. The most significant differences between homologous X-chromosomes were found for NX-E1, NX-E2 and E1-E2 distances that were in average about twice longer for XL as compared with XS. The longer E1-E2 distances for XL indicate more open chromatin structure of the dystrophin gene on this chromosome in contrary to closed structure on XS.
Trvalý link: http://hdl.handle.net/11104/0025095