Chemiluminescence at the Turn of the Millennium. An Indispensible Tool in modern Chemistry, Biochemistry and Medicine

0126504 - BFU-R 20013064 RIV DE eng M - Část monografie knihy
Souček, Karel - Kubala, Lukáš - Lojek, Antonín - Kozubík, Alois
Analysis of Reactive Oxygen Species Production and Myeloperoxidase Activity in Chemically Induced Differentiation of Human Myeloid Leukemia Cells.
Chemiluminescence at the Turn of the Millennium. An Indispensible Tool in modern Chemistry, Biochemistry and Medicine. Dresden: SCHWEDAWERBEDRUCK GmbH, Druckerei and Verlag, 2001 - (Albrecht, S.; Zimmermann, T.; Brandl, H.), s. 156-161. ISBN 3-9807853-0-0
Grant CEP: GA ČR GA524/99/0694; GA AV ČR IBS5004009
Výzkumný záměr: CEZ:AV0Z5004920
Klíčová slova: chemiluminescence * flow cytometry * leukemia cells
Kód oboru RIV: BO - Biofyzika

In our study, Hl-60 cells differentiated into neutrophil-like or monocyte-like cells were used. The total ROS production was measured using luminol-enhanced chemiluminescence. Intracellular production of ROS was determinated by flow cytometric analysis using dichlorofluorescein diacetate, dihydrorhodamine, and hydroethidine. MPO activity was detected spectrophotometrically using o-dianisidine, and by the CL method based on bromide-dependent CL
Trvalý link: http://hdl.handle.net/11104/0024732