Transposition of IS10 from the host Escherichia coli genome to a plasmid may lead to cloning artefacts

0126465 - BFU-R 20013025 RIV DE eng J - Článek v odborném periodiku
Kovařík, Aleš - Matzke, M. A. - Matzke, A. J. M. - Koukalová, Blažena
Transposition of IS10 from the host Escherichia coli genome to a plasmid may lead to cloning artefacts.
Molecular Genetics and Genomics. Roč. 266, č. 2 (2001), s. 216-222. ISSN 1617-4615. E-ISSN 1617-4623
Grant CEP: GA ČR GA521/01/0037; GA AV ČR IBS5004010
Výzkumný záměr: CEZ:AV0Z5004920
Klíčová slova: IS10 transposition * IS elements * DNA cloning
Kód oboru RIV: BO - Biofyzika

The present paper describes and example of bacterial IS10 transposition from genome to a plasmid during cloning steps in a laboratory. The database search revealed unusually high frequency of IS10 elements in cloned eukaryotic sequences. It is estimated that approximately every thousandth clone in a database contains an IS contamination. We recommend checking submitted sequences for the presence of IS10 and other IS elements. In addition, DNA databases should be corrected by removing contaminating IS sequences
Trvalý link: http://hdl.handle.net/11104/0024694