Localization of Male-Specifically Expressed MROS Genes of Silene latifolia by PCR on Flow-Sorted Sex Chromosomes and Autosomes

0126464 - BFU-R 20013024 RIV US eng J - Článek v odborném periodiku
Kejnovský, Eduard - Vrána, Jan - Matsunaga, S. - Souček, Přemysl - Široký, Jiří - Doležel, Jaroslav - Vyskot, Boris
Localization of Male-Specifically Expressed MROS Genes of Silene latifolia by PCR on Flow-Sorted Sex Chromosomes and Autosomes.
Genetics. Roč. 158, č. 3 (2001), s. 1269-1277. ISSN 0016-6731. E-ISSN 1943-2631
Grant CEP: GA ČR GV521/96/K117; GA ČR GA521/99/0696; GA MŠMT ME 380
Výzkumný záměr: CEZ:AV0Z5004920
Klíčová slova: Plant Melandrium-album * dioecious Silene * Y-chromosome
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 4.803, rok: 2001

To localize the MROS (male-specifically expressed genes) in the dioecious Silene latifolia (white campion) flow-sorted chromosomes were used as a template for PCR with specific primers. Our results indicate that the MROS3 gene is located in at least two copies tandemly arranged on the X chromosome with additional copies on the autosomes, while MROS1, MROS2, and MROS4 are exclusively autosomal. Homology search of databases has shown the presence of five MROS3 homologues in Arabidopsis thaliana
Trvalý link: http://hdl.handle.net/11104/0024693