Chitinase isozymes induced by TYMV and Leptosphaeria maculans during compatible and incompatible interaction with Brassica napus

0099891 - ÚEB 2008 RIV NL eng J - Článek v odborném periodiku
Profotová, Bronislava - Burketová, Lenka - Valentová, O.
Chitinase isozymes induced by TYMV and Leptosphaeria maculans during compatible and incompatible interaction with Brassica napus.
[Isoenzymy chitinas indukované TYMV a Leptosphaeria maculans při kompatibilní a inkompatibilní interakci u Brassica napus.]
Biologia Plantarum. Roč. 51, č. 3 (2007), s. 507-513. ISSN 0006-3134. E-ISSN 1573-8264
Grant CEP: GA ČR GA522/03/0353
Výzkumný záměr: CEZ:AV0Z50380511
Zdroj financování: V - jiné veřejné zdroje
Klíčová slova: Chitinase * TYMV * Leptosphaeria maculans * Brassica napus * PR-proteins
Kód oboru RIV: GF - Choroby, škůdci, plevely a ochrana rostlin
Impakt faktor: 1.259, rok: 2007

Accumulation of extracellular chitinases in Brassica napus plants infected with Turnip yellow mosaic virus (TYMV) and fungal pathogen Leptosphaeria maculans was studied in both compatible and incompatible interaction. Analysis of apoplast fluid by means of non-denaturing anodic and cathodic PAGE followed by in-gel detection of chitinase activity revealed a number of chitinase isozymes. TYMV induced 8 acidic and 4 basic isozymes in a systemic way. Except for one acidic and one basic isozyme, all other chitinases were also constitutively present in low amounts in mock inoculated control. In TYMV systemically infected plants, chitinases were detected in leaves expressing symptoms as well as in symptomless ones. Both virulent and avirulent L. maculans isolates induced production of chitinase isozymes in cotyledons in a time dependent manner. Some of them were present in plants constitutively and their content increased after inoculation. Three of five acidic and two of three basic isozymes responded to L. maculans infection. Chitinases started to accumulate before symptom appearance. First two acidic isozymes were detected 24 h after inoculation. The difference between compatible and incompatibe interaction reflected two basic isozymes.

V listech Brassica napus infikovaných virem žluté mozaiky vodnice a houbovým patogenem Leptosphaeria maculans se akumulují extracelulární chitinasy při inkompatibilní i kompatibilní interakci. Proteiny apoplastu byly analyzovány pomocí PAGE za nativních podmínek a v gelu detekovány kyselé i bazické isoenzymy chitinas.
Trvalý link: http://hdl.handle.net/11104/0158361