DNA damage in potato plants induced by cadmium, ethyl methanesulphonate and gama-rays

0099655 - ÚEB 2008 RIV NL eng J - Článek v odborném periodiku
Gichner, Tomáš - Patková, Zdeňka - Száková, J. - Žnidar, I. - Mukherjee, A.
DNA damage in potato plants induced by cadmium, ethyl methanesulphonate and gama-rays.
[Poškození DNA u rostlin brambor po aplikaci kadmia, ethyl methansulfonátu a γ- paprsků.]
Environmental and Experimental Botany. Roč. 62, č. 2 (2008), s. 113-119. ISSN 0098-8472. E-ISSN 1873-7307
Grant CEP: GA ČR GA521/05/0500
Výzkumný záměr: CEZ:AV0Z50380511
Zdroj financování: V - jiné veřejné zdroje
Klíčová slova: Comet assay * Solanum tuberosum L.
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.301, rok: 2008

We have calibrated the alkaline protocol of the plant comet (Single Cell Gel Electrophoresis) assay as a method for detecting the extent of induced DNA damage in potato plants (Solanum tuberosum L. cultivar Korela). After 2 and 24 h treatments of the rooted cuttings with the heavy metal cadmium (Cd2+), a dose–response increase in DNA damage was noted versus controls in root nuclei. With a 24 h recovery period, the Cd2+-induced DNA damage in roots increased significantly. No significant increase in DNA damage was demonstrated in leaf nuclei after 24 h Cd2+ treatments, but continuous Cd2+ treatments for 2 weeks resulted in an increase in leaf DNA damage. This increase may be however associated with necrotic and apoptotic DNA fragmentation, as the affected plants had inhibited growth and distorted yellowish leaves. For comparison, the monofunctional alkylating agent ethyl methanesulphonate, and γ-rays were assessed for induced DNA damage. Analysis of the accumulation of cadmium by inductively coupled plasma optical emission spectrometry demonstrates that roots accumulate almost 9-fold more cadmium than aboveground parts of the rooted potato cuttings. This may explain the absence of Cd2+ genotoxicity in leaves after short-term treatments.

Zakořeněné řízky brambor Solanum tuberosum kultivar Korela byly ovlivněny těžkým kovem kadmiem. Poškození DNA měřeno technikou komet, bylo prokázáno jen v kořenech rostlin brambor, zatímco v listech ke zvýšení poškození DNA nedošlo. Analýza obsahu kadmia za použití atomového absorpčního spektrometru prokázalo, že kořený akumulují až 9x více kadmia než listy. Po aplikaci ethyl methansulfonátu a γ- paprsků došlo k poškození DNA jak v kořenech tak v listech.
Trvalý link: http://hdl.handle.net/11104/0158206