Anatomical and biochemical studies of anthocyanidins in flowers of Annagalis monelli L. (Primulaceae) hybrids

0091003 - BÚ 2008 RIV NL eng J - Článek v odborném periodiku
Quintana, A. - Albrechtová, Jana - Griesbach, R. J. - Freyre, R.
Anatomical and biochemical studies of anthocyanidins in flowers of Annagalis monelli L. (Primulaceae) hybrids.
[Anatomické a biochemické studie anthokyanidinů v hybridních květinách Annagalis monelli L. (Primulaceae).]
Scientia horticulturae. Roč. 112, č. 4 (2007), s. 413-421. ISSN 0304-4238. E-ISSN 1879-1018
Grant CEP: GA AV ČR(CZ) IAA600110507
Výzkumný záměr: CEZ:AV0Z60050516
Klíčová slova: flower color * epidermis * structural study
Kód oboru RIV: EF - Botanika
Impakt faktor: 0.694, rok: 2007

Violet, lilac and red flower colors segregated in an F3 population obtained from hybridizing blue and orange breeding lines of Anagallis monelli at UNH. One individual per color was studied, as well as true-blue cultivar Skylover Blue. Anatomical examination revealed typical petal layout with upper epidermis, loose mesophyll and lower epidermis. Cells in upper and lower epidermis were categorized by their vacuole color. Blue and red individuals had mostly blue and red cells, respectively. Lilac and violet individuals had blue and bicolored (red and blue) cells on both surfaces, and red cells on the lower epidermis only. Violet individuals had more blue cells on the upper epidermis than lilac individuals.Anthocyanidins were determined by HPLC for each petal epidermis. Blue flowers had only malvidin in both petal surfaces, red flowers had mostly delphinidin with traces of malvidin. Lilac and violet flowers had more malvidin than delphinidin. For violet and lilac flowers respectively, 2 and 3% delphinidin in upper petal surfaces result in a reddish tone while in the lower surface 33 and 25% delphinidin result in a red color. pH in upper and lower petal surfaces were significantly different for each individual, which may affect final flower color.

Studie byla prováděna na F3 populaci hybridizací modré a oranžové linie Annagalis monelli na university of New Hampshire za účelem zjištění jaká je biochemická a strukturální podstata různé barevnosti okvětních plátků hybridních rostlin A. monelli. Anatomická studie ukázala rozdíly v lokalizaci anthokyanidinů v buňkách svrchní a spodní epidermis okvětních lístků. HPLC studie obou epidermis okvětních lístků prokázala přítomnost malvidinu a delfidinu. Též bylo zjištěno odlišné pH, které též může ovlivňoat výsledné zbarvení okvětních plátků.
Trvalý link: http://hdl.handle.net/11104/0151709