Eukaryotic-Type Serine/Threonine Protein Kinase StkP Is a Global Regulator of Gene Expression in Streptococcus pneumoniae

0084887 - MBÚ 2008 RIV SIGLE US eng J - Článek v odborném periodiku
Přenosilová, Lenka - Nováková, Linda - Basler, Marek - Branny, Pavel
Eukaryotic-Type Serine/Threonine Protein Kinase StkP Is a Global Regulator of Gene Expression in Streptococcus pneumoniae.
[Serin-threoninová proteinkinasa StkP jako globální regulátor genové exprese Streptococcus pneumoniae.]
Journal of Bacteriology. Roč. 189, č. 11 (2007), s. 4168-4179. ISSN 0021-9193. E-ISSN 1098-5530
Grant CEP: GA ČR GP204/07/P082; GA ČR GA204/02/1423; GA MZe 1G46068
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: protein kinase * streptococus pneumoniae * gene expression
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 4.013, rok: 2007

Signal transduction pathways in both prokaryotes and eukaryotes utilize protein phosphorylation as a key regulatory mechanism. Recent studies have proven that eukaryotic-type serine/threonine protein kinases (Hanks-type) are widespread in many bacteria, although little is known regarding the cellular processes they control. In this study, we have attempted to establish the role of a single eukaryotic-type protein kinase, StkP of Streptococus pneumoniae, in bacterial survival. Our results indicate that expression of StkP is important for the resistance of S. pneumoniae to various stress conditions. To investigate the impact of StkP on this phenotype, we compared the whole-genome expression profiles of the wild type and ΔstkP mutant strains by microarray technology. This analysis revealed that StkP positively controls transcription of a set of genes encoding functions involved in cell wall metabolism, pyrimidine biosynthesis, DNA repair, iron uptake and oxidative stress response

Tato studie se zabývá analýzou funkce proteinkinasy eukaryotního typu StkP u Streptococcus pneumoniae. Bylo zjištěno, že exprese StkP je důležitá pro rezistenci S. pneumoniae k různým druhům stresů. Dále byly porovnány expresní profily kmenů divokého typu a mutanta ΔstkP a bylo prokázáno, že StkP kontroluje expresi genů kódujících proteiny rozličné funkce
Trvalý link: http://hdl.handle.net/11104/0147521