Allelic variation in porcine resistin (RETN) gene is associated with fatness traits in a Wild Boar x Meishan reference family

0054487 - ÚŽFG 2007 RIV BR eng C - Konferenční příspěvek (zahraniční konf.)
Čepica, Stanislav - Knoll, A. - Masopust, Martin - Vykoukalová, Z. - Bartenschlager, H. - Nonneman, D. - Rohrer, G. A. - Geldermann, H.
Allelic variation in porcine resistin (RETN) gene is associated with fatness traits in a Wild Boar x Meishan reference family.
[Alelická variabilita prasečího resistinového (RETN ) genu je asociována s ukládáním tuku u referenční rodiny divoké prase x meishan.]
Proceedings of the 30th International Conference on Animal Genetics ISAG 2006. Porto Seguro: ISAG, 2006, s. 72. ISBN 85-85584-02-5.
[30th International Conference on Animal Genetics. Porto Seguro (BR), 20.08.2006-25.08.2006]
Grant CEP: GA ČR GA523/04/0106
Výzkumný záměr: CEZ:AV0Z50450515
Klíčová slova: RETN gene
Kód oboru RIV: EB - Genetika a molekulární biologie

Cloning and comparative sequencing of the porcine resistin (RETN) gene and 5’ flanking region, located at 64 cM on SSC2, revealed 9 SNPs and 2 indels. A European Wild Boar x Meishan family encompassing 335 F2 animals measured for 46 traits including growth, fat deposition and muscle accretion was scored for a T2094C polymorphism by means of MvaI PCR-RFLP. Two haplotypes (AM157180 and AM157181) segregated in the family while the imprinted QTL in IGF2 was fixed. The founder boar was homozygote MvaI BB while Meishan F0 sows segregated alleles MvaI A and MvaI B. Previous QTL mapping by Lee et al. (2003) did not reveal any QTL for fat accretion in the MYOD - SW395 interval where RETN is located. Association analysis showed that the MvaI A allele of Meishan origin accompanied by the 1472A allele encoding for 36Ala is associated with higher values for fat deposition traits (fat depth at 10th rib, back fat depth at 13th/14th rib, loin fat depth, average back fat depth, back fat weight, ham subcutaneous fat weight, fat cuts, fat area at 13th/14th rib, shoulder subcutaneous fat weight, shoulder fat depth), growth rate (live weight at slaughter, half carcass weight), fat to meat ratio at 13th/14th rib, food consumption from 110 to 210 day and number of teats, as compared to allele MvaI B coupled with 1472G allele encoding for 36Thr. Allelic variation within the RETN gene accounts for six percent of phenotypic variation of average back fat depth and fat depth at 10th rib. The associations measured between RETN variants and fat deposition traits indicate mainly additive gene effects. Further experiments are needed to elucidate whether the association between alleles and trait values is caused by the nonsynonymous G1473A mutation causing the Ala36Thr amino acid substitution, allelic variation in the promoter region or by DNA variants in closely linked genes.

Klonováním a komparativním sekvenováním prasečího resistinového genu, lokalizovaného na v pozici 64 na SSC2, cM a 5’ přiléhající oblasti bylo zjištěno 9 SNP a indely. Rodina divoké prase x meishan mající 335 F2 potomků, u kterých bylo zjišťováno 46 vlastností (včetně růstu, ukládání tuku a růstu svalů) a byli pomocí MvaI PCR-RFLP testováni na polymorfismus T2094C . V rodine segregovaly dva haplotypy (AM157180 a AM157181)zatím co imprintovaný QTL v IGF2 byl fixován.
Trvalý link: http://hdl.handle.net/11104/0142551