Transmission of Mycobacterium tuberculosis Undetected by Tuberculin Skin Testing

0039366 - MBÚ 2007 RIV SIGLE US eng J - Článek v odborném periodiku
Anderson, S. T. - Williams, A. J. - Brown, J. R. - Newton, S. M. - Šimšová, Marcela - Nicol, M. P. - Šebo, Peter - Levin, M. - Wilkinson, R. J. - Wilkinson, K. A.
Transmission of Mycobacterium tuberculosis Undetected by Tuberculin Skin Testing.
[Přenos Mycobacterium tuberculosis nedetekovaný kožním tuberkulinovým testem.]
American Journal of Respiratory and Critical Care Medicine. Roč. 173, - (2006), s. 1038-1042. ISSN 1073-449X. E-ISSN 1535-4970
Grant CEP: GA AV ČR IAA5020406
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: adenylate cyclase * diagnostic tests and procedures * mycobacterium tuberculosis
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 9.091, rok: 2006

Rationale: The development of tuberculin skin test (TST) positivity following infection by Mycobacterium tuberculosis is not invariable and may depend on bacillary as well as host factors. Objectives: First, to compare the diagnostic performance of the TST and a form of in vitro IFN-gamma release assay (IFNGRA) in the circumstances of a contact investigation prompted by an unusually severe index case of infectious pulmonary tuberculosis. Second, to investigate the ability of the strain of M. tuberculosis responsible to induce cytokine secretion from monocytes in vitro. Methods: A routine TST-based tuberculosis-contact screening procedure supplemented by the use of an "in house" IFNGRA that assays the T-cell response to the M. tuberculosis-specific antigens ESAT-6, CFP-10 (presented as a fusion protein within the inactivated adenylate cyclase of Bordetella pertussis), and purified protein derivative of M. tuberculosis

Práce ukazuje, že tuberkulinový kožní test, tak jak byl používán v UK (Heaf test) není spolehlivý a nedetekuje infekce atypickými kmeny Mycobacterium tuberculosis. V této práci popisujeme použití nově vyvinutého testu založeného na použití detekce in vitro uvolněného IFNg
Trvalý link: http://hdl.handle.net/11104/0133473