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NANODIAMONDS ENCAPSULATED IN NANOFIBERS AS A NEW STRATEGY FOR MICRORNA-BASED THERAPY OF PROSTATE CANCER
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SYSNO ASEP 0522949 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název NANODIAMONDS ENCAPSULATED IN NANOFIBERS AS A NEW STRATEGY FOR MICRORNA-BASED THERAPY OF PROSTATE CANCER Tvůrce(i) Bitti, Giuseppe (UEM-P)
Divín, R. (CZ)
Benson, Veronika (MBU-M) RID, ORCID
Filová, Eva (UEM-P) RID, ORCID
Amler, E. (CZ)Zdroj.dok. NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application. - Ostrava : Tanger Ltd., 2019 - ISBN 978-808729489-5 Rozsah stran s. 348-353 Poč.str. 6 s. Forma vydání Tištěná - P Akce NANOCON 2018 -International Conference on Nanomaterials - Research and Application /10./ Datum konání 17.10.2018 - 19.10.2018 Místo konání Brno Země CZ - Česká republika Typ akce EUR Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova nanodiamonds ; nanofibers ; gene therapy Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Biomaterials (as related to medical implants, devices, sensors) Vědní obor RIV – spolupráce Mikrobiologický ústav - Biotechnologie a bionika CEP LO1309 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LO1508 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEM-P - RVO:68378041 ; MBU-M - RVO:61388971 UT WOS 000513131900060 EID SCOPUS 85062984757 Anotace Given the miRNA-34 family role as tumor suppressor in cancer, it was showed that using miRNA-34a as a new anticancer agent has a great therapeutic potential. Unfortunately, biopharmaceutical issues, rapid degradation in biological fluids, poor uptake into cells and not specific distribution into the body, prevent the use of wildtype non-coding RNA by systemic administration. To overcome these limits, in our previous study we developed a new therapeutic concept based on nanotechnology delivery of miRNA-34a into prostate cancer using fluorescent nanodiamond particles coated with polyethylenimine. In the current study, in order to achieve a more conservative, stable and controlled release of miRNA-34a, we designed a system based on nanodimaonds- miRNA34a complexes encapsulated into nanofibers. We successfully encapsulate nanodiamonds into fibers of polycaprolactione, a biodegradable polymer with a slow degradation rate and a high cellular adhesion, using an electropsinning process. We cultivated prostate cancer cells (PC3 and DU145) on nanofibers scaffolds and we analyzed the presence of nanodiamonds in cells using confocal microscopy. Moreover, in these prostate cancer cell lines we observed a replacement of miRNA-34a via real-time PCR and a decreased viability testing the metabolic activity. Our preliminar results suggest a higher increased and stable mid-term replacement of oncosuppressor miRNA-34a in prostate cancer when the nanodiamonds-miRNA complexes are delivered encapsulating them in nanofibers. Therefore we provided an effective strategy for cancer therapy ready to be tested for in vivo experiments. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2020
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