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Endogenous retroviral insertions drive non-canonical imprinting in extra-embryonic tissues
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SYSNO ASEP 0518783 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Endogenous retroviral insertions drive non-canonical imprinting in extra-embryonic tissues Tvůrce(i) Hanna, C. W. (GB)
Peréz-Palacios, R. (FR)
Gahurová, Lenka (UZFG-Y) ORCID
Schubert, M. (NL)
Krueger, F. (GB)
Biggins, L. (GB)
Andrews, S. (GB)
Colomé-Tatché, M. (NL)
Bourc´his, D. (NL)
Dean, W. (GB)
Kelsey, G. (GB)Číslo článku 225 Zdroj.dok. Genome Biology - ISSN 1474-760X
Roč. 20, č. 1 (2019)Poč.str. 17 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova genomic imprinting ; histone modifications ; extra-embryonic Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemistry and molecular biology Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000502825300001 EID SCOPUS 85074346124 DOI 10.1186/s13059-019-1833-x Anotace Background: Genomic imprinting is an epigenetic phenomenon that allows a subset of genes to be expressed mono-allelically based on the parent of origin and is typically regulated by differential DNA methylation inherited from gametes. Imprinting is pervasive in murine extra-embryonic lineages, and uniquely, the imprinting of several genes has been found to be conferred non-canonically through maternally inherited repressive histone modification H3K27me3. However, the underlying regulatory mechanisms of non-canonical imprinting in postimplantation development remain unexplored.
Results: We identify imprinted regions in post-implantation epiblast and extra-embryonic ectoderm (ExE) by assaying allelic histone modifications (H3K4me3, H3K36me3, H3K27me3), gene expression, and DNA methylation in reciprocal C57BL/6 and CAST hybrid embryos. We distinguish loci with DNA methylation-dependent (canonical) and independent (non-canonical) imprinting by assaying hybrid embryos with ablated maternally inherited DNA methylation. We find that non-canonical imprints are localized to endogenous retrovirus-K (ERVK) long terminal repeats (LTRs), which act as imprinted promoters specifically in extra-embryonic lineages. Transcribed ERVK LTRs are CpG-rich and located in close proximity to gene promoters, and imprinting status is determined by their epigenetic patterning in the oocyte. Finally, we show that oocyte-derived H3K27me3 associated with non-canonical imprints is not maintained beyond pre-implantation development at these elements and is replaced by secondary imprinted DNA methylation on the maternal allele in post-implantation ExE, while being completely silenced by bi-allelic DNA methylation in the epiblast.
Conclusions: This study reveals distinct epigenetic mechanisms regulating non-canonical imprinted gene expression between embryonic and extra-embryonic development and identifies an integral role for ERVK LTR repetitive elements.Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2020 Elektronická adresa https://genomebiology.biomedcentral.com/articles/10.1186/s13059-019-1833-x
Počet záznamů: 1